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Los talleres fueron convocados por la Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO), el Instituto Nacional de Estadística, Geografía e Informática (INEGI) y el Instituto Nacional de Ecología (INE). La reunión bilateral se realizó entre miembros de la CONABIO y del INEGI para efectuar ajustes a algunas de las ecorregiones. El primer taller de expertos fue realizado los días 24 y 25 de febrero de 2005, y el segundo el 30 de marzo de 2005, ambos en las instalaciones de CONABIO en la ciudad de México. Se tomaron como puntos de partida dos mapas de ecorregiones: el de la Comisión para la Cooperación Ambiental (CCA) nivel III (1997) y el de la World Wild Foundation (WWF)-CONABIO-CCA (1997); teniendo como objetivo llegar a un consenso entre los expertos en la definición y delimitación de ecorregiones y producir un mapa único a escala 1:1000 000. 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